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Cell Culture and Cell Isolation

Trypsin

GIBCO® trypsin solutions are used widely for dissociation of tissues and cell monolayers and we can deliver solutions that will perform consistently, batch after batch.

The biochemical assays performed on Trypsin 1:250 determine both trypsin specific-activity at the level of certain co-purified enzymes that influence cell removal and viability.  All trypsin solutions are prepared from porcine-parvovirus-tested and mycoplasma-tested materials.

Lastly, GIBCO® trypsin solutions are prepared under controlled conditions using accelerated manufacturing procedures to minimize loss of enzyme activity from denaturation and autodegradation.
Trypsin and Trypsin EDTA Products
Other Cell Dissociation Reagents
TrypLE™ Express - Trypsin Replacement Enzyme
TrypLE™ Express:  the superior replacement for trypsin

  • Gentle on cells - higher plating efficiency
  • Saves time - eliminates the need to stagger harvesting
  • Room temperature stable - ready to use when you need it
  • Easy to use - directly substitutes into existing protocols

Choose the Proper Cell Dissociation Product

Culture TypeWash SolutionDissociation Solution
Products and Cat. No.
Most continuous and strongly adherent cell lines




Many cells at early passage
HBSS or PBS w/o calcium and magnesium




D-PBS
Trypsin 0.25% in balanced salt solution w/o calcium and magnesium



TrypLE™ products
0.25% Trypsin (liquid) (1X) 15050-065
2.5% Trypsin (liquid) (10X) 15090-046
Trypsin Powder (1:250) 27250-018

TrypLE™ Select (liquid) 12563-011
TrypLE™ Express (liquid) 12605-010
Continuous cell lines where cell surface protein integrity is importantHBSS or PBS w/o calcium and magnesiumTrypsin 0.05% in 0.53 mM EDTA



TrypLE™ products
0.05% Trypsin 0.53 mM EDTA (liquid) 25300-054
0.05% Trypsin 0.53 mM EDTA (liquid) (10X) 15400-054

TrypLE™ Select (liquid) 12563-011
TrypLE™ Express (liquid) 12605-010
Weakly adherent epithelial cells



Transformed fibroblasts

Primary cells where cell surface protein integrity is important
HBSS or PBS w/o calcium and magnesiumEDTA, glycerol in sodium citrate



TrypLE™ products
Enzyme-Free Cell Dissociation Buffers, Hanks' based 13150-016, PBS based 13151-014

TrypLE™ Select (liquid) 12563-011
TrypLE™ Express (liquid) 12605-010
Strongly adherent early-passage cell linesHBSS or PBS w/o calcium and magnesiumTrypsin 0.25% in 1 mM EDTA



Dispase 0.6 to 2.4 units/ml in PBS
0.25% Trypsin 1 mM EDTA (liquid) 25200-056
Trypsin Powder (1:250) 27250-018

Dispase
Epithelial cells0.5 mM to 1 mM EDTA0.5 mM to 1 mM EDTA


Dispase 0.6 to 2.4 units/ml in PBS
Versene 1:5,000 (0.53 mM EDTA in PBS)

Dispase
Strongly adherent, epithelial and some tumor cell lines
0.5 mM to 1 mM EDTA0.25% with 1 mM EDTA






Dispase 0.6 to 2.4 units/ml in PBS w/o calcium and magnesium
0.25% Trypsin 1 mM EDTA (liquid) 25200-056
Versene 1:5,000 (0.53 mM EDTA in PBS)
Trypsin Powder (1:250) 27250-018

Dispase
Thick cultures, multiple layers



Dense cultures that are collagen rich
1 mM EDTATrypsin 0.25%




Collagenase 200 units/ml, 1 mM EDTA in balanced salt solution w/o calcium and magnesium
0.25% Trypsin 1 mM EDTA (liquid) 25200-056
Trypsin Powder (1:250) 27250-018

Collagenase Type I, Type II, Type IV


All adherent cell culturesHBSS or PBS w/o calcium and magnesiumScrape off cell sheet
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