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cloning

Select the correct TOPO® Cloning Kit by Application

Whether you’re PCR cloning with Taq DNA polymerase or a proofreading enzyme, there is a TOPO® cloning kit available to take you quickly and efficiently into your downstream application (Table 2).
Application of cloned PCR product Product Type of TOPO® cloning Advantages One Shot® Competent CellsCat. no.
General subcloning TOPO® TA Cloning® Kit TA
  • Get your clone 95% efficiency
  • Selection of competent cells for high speed, electroporation, or routine cloning
  • Mach1-T1R K451020
    TOP10 K450001
    TOP10F’ K455001
    TOP10 Electrocomp™ K456001
    MAX Efficiency® DH5α™-T1 Phage-Resistant K452001
    Zero Blunt® TOPO® PCR Cloning Kit Blunt
  • Unique technology minimizes background
  • Get your clone 95% efficiency
  • Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convinient
  • Selection of competent cells for high speed, electroporation, or routine cloning
  • Mach1™-T1R K283020
    TOP10 K280020
    TOP10 Electrocomp™ K286020
    MAX Efficiency® DH5α™-T1 Phage-Resistant K282020
    Long PCR fragments TOPO® XL PCR Cloning Kit TA
  • Combining TOPO®, ZeroBackground™ and Crystal Violet technologies makes for easy cloning of large fragments 3-10 kb.
  • Unique technology to minimize background
  • Mach1™-T1R K703020
    TOP10 K475010
    TOP10 Electrocomp™ K470010
    In vitro transcription TOPO® TA Cloning® Kit Dual Promoter TA
  • Get your clone 95% efficiency
  • Dual T7 and SP6 priming sites for in vitro transcription
  • Mach1™-T1R K461020
    TOP10F’ K460001
    TOP10 Electrocomp™ K466001
    MAX Efficiency® DH5α™ -T1 Phage-Resistant K462001
    Sequencing TOPO® TA Cloning® Kit for Sequencing  (pCR® 4 TOPO® Vector) TA
  • Get your clone 95% efficiency
  • Sequence more insert, less vector
  • Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convinient 
  • Mach1™-T1R K453020
    TOP10 K457501
    TOP10 Electrocomp™ K458001
    MAX Efficiency® DH5α™ -T1 Phage-Resistant K289520
    Zero Blunt® TOPO® PCR Cloning Kit for Sequencing (pCR® 4 Blunt TOPO® Vector) Blunt
  • Unique technology minimizes background
  • Get your clone 95% efficiency
  • Sequence more insert, less vector
  • Mach1™-T1R K283520
    TOP10 K287520
    MAX Efficiency® DH5α™-T1 Phage-Resistant K289520
    TOP10 Electrocomp™ K288020
    Expression in E. coli Champion™ pET100 Series Directional TOPO® Expression Kits (pET Directional TOPO® Vector)Directional
  • Up to 90% of clones in the correct orientation
  • High-level protein expression using E. coli
  • Convenient purification and detection tags 
  • BL21 Star™(DE3) K10001
    K10101
    K10201
    K15101
    Expression in mammalian cells pcDNA™ 3.3 TOPO® TA Cloning® Kit TA
  • 3–5 higher expression compared to conventional CMV vectors
  • Ideal for expression of native protein
  • TOP10 K830001
    pcDNA™ 3.2/V5/GW/D-TOPO® Cloning Kit Directional
  • Greater than 90% of clones in correct orientation
  • CMV promoter delivers high-level expression in mammalian cells
  • C-terminal V5 and 6xHis tags for convenient detection and purification
  • Neomycin selection
  • TOP10 K244020
    ViraPower™ HiPerform™ Lentiviral FastTiter™ TOPO® Expression Kits (pLenti 7.3 Vector)TA
  • Greater than 4X higher expression compared to the original system
  • Efficient TOPO® cloning
  • Stbl3™K495000
    Entry into Gateway® Entry VectorspCR®8/GW/TOPO® TA Cloning® Kit TA
  • Includes Plasmid miniprep kit
  • Fast growing cells for fast miniprep
  • Mach1™-T1R K532000
    TA
  • Fast growing cells for fast miniprep
  • Mach1™-T1R K252020


    TOP10 K250020
    pENTR™/TEV/DTOPO® Cloning Kit Directional
  • Fast growing cells for fast miniprep
  • Mach1™-T1R K252520
    pENTR™/D-TOPO® Cloning Kit Directional
    TOP10 K240020
    pENTR™/SD/DTOPO® Cloning Kit Directional
    TOP10 K242020
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